To this purpose, during recent years, amino acids are gaining interest as a mean factor for the nutrition-mediated improvement of the immune system. Proper nutrition is considered as one of the most important factors in maintaining a functional immune system and preventing inflammation-inducing conditions such as tissue damage, metabolic syndrome-related disorders, etc. However, the enhancement of animals’ immune system the last years can be achieved by alternative nutritional strategies than that of antibiotics, such as the use of amino acids etc. A strengthen of animals’ immune system is close related with farm sustainability.
The intensive farming of high genetic merit ruminants can be associated with several issues including immunosuppression, which makes them susceptible to infections with negative consequences in their wellbeing and production. In conclusion, our results suggest that the dietary supplementation of ewes with rumen-protected amino acids, down regulate the expression of some genes involved in the pro-inflammatory signalling.
Lower transcript levels of STAT-3 gene were observed in the neutrophils of ewes fed with either C or with E diets in comparison with the control. The mRNA relative expression levels of JunD gene were highly induced in the monocytes, while those of IL-10 and HO-1 genes were declined in the neutrophils of ewes fed with the C and D diets compared with the control. Significantly lower mRNA transcript accumulation was also observed for the MyD88 gene in the neutrophils of ewes fed with lysine only (C). The results revealed a significant downregulation of relative transcript level of the IL-1α gene in the neutrophils of C and in monocytes of D ewes compared with the control. Thus, sixty Chios dairy ewes ( Ovis aries) were assigned to one of the following five dietary treatments (12 animals/ treatment): A: basal diet consist of concentrates, wheat straw and alfalfa hay (control group) B: basal diet +6.0 g/head rumen-protected methionine C: basal diet + 5.0 g/head rumen-protected lysine D: basal diet +6.0 g/head rumen-protected methionine + 5.0 g/head rumen-protected lysine and E: basal diet +12.0 g/head rumen-protected methionine + 5.0 g/head rumen-protected lysine. The objective of this work was to determine the impact of dietary supplementation with rumen-protected methionine or lysine, as well as with a combination of these amino acids in two different ratios, on the expression of selected key-genes (NLRs, MyD88, TRIF, MAPK-1, IRF-3, JunD, TRAF-3, IRF-5, IL-1α, IL-10, IKK-α, STAT-3 and HO-1).
Rumen protected amino acids inclusion in ewes’ diets has been proposed to enhance their innate immunity.
0 kommentar(er)
